The seed DNA extraction instrument played an important role in this research. The extraction of the seed DNA of Perilla frutescens was based on the improved CTAB method for extraction of tobacco DNA, with slight modifications. The reagents were all domestic analytically pure. Take 0.1~0.2g seeds, place them in a mortar cooled at -20°C, and grind them into a 1.5mL centrifuge tube. Add 0.35mL of 1×DNA preheated 55°C extract (50mmol). /LTris-HCl pH7.5, 10mmol/LEDTApH8.0, 0.7mmol/L NaCl, 1% β-mercaptoethanol, 1% CTAB), 3 min later, 2× DNA extract (concentration twice as much as the former) was placed in 55 Incubate overnight in the incubator, invert several times between them, add 0.7mL saturated phenol, mix and extract for 3h on the homemade mixer (2r/min), centrifuge for 10min at 6000r/min, transfer the supernatant to another In a tube, 0.35 mL of saturated phenol and 0.35 mL of chloroform-isoamyl alcohol (24:1) were extracted for 2 h, centrifuged at 6000 r/min for 10 min, the supernatant was taken to another tube, and 0.7 mL of chloroform-isopentyl was added. Alcohol (24:1) was extracted for 2 h, centrifuged at 6000r/min for 10 min, supernatant was taken to another tube, 0.07 mL of 3 mol/L NaAc and 0.7 mL of isopropanol were added, and precipitated at room temperature for 30 min and centrifuged at 3000 r/min for 5 min. The supernatant was discarded and the DNA was washed with 70% alcohol, dried, and then dissolved in 100 μ LTE.
In the study of plant molecular markers, the genomic DNA extracted from the seed DNA extractor generally uses fresh young leaves as a material, and rarely using mature seeds as materials for the extraction of genomic DNA. Since perilla leaves are rich in perilla pigments and phenolic substances, it is difficult to obtain high-quality genomic DNA without special treatment. Therefore, we tried to extract the genomic DNA of Perilla frutescens with modified CTAB method using perilla seed as material. .
B. Main particulars
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MAIN PARTICULARS |
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Material |
Casting iron, Bronze(C51000, C52100, C54400-), Powder metal, Plastic |
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Standard |
ASTM, ASME, DIN, JIS, ISO, BS, API, EN |
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Certificate |
ISO9001 |
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Tolerance |
Up to +/- 0.002mm |
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Dimension |
As per drawing, as large as we can |
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Processing |
CNC turning, CNC turning and milling compound processing, 3/4/5 axis CNC machining center, wire-cutting, EDM, grinding etc. |
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Finishing surface |
Machined surface with oil, blasting and painting, anodize, polishing, powder painting, electrophoretic coating- |
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Marking |
as per clients` requirements |
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Inspection |
material, construction, dimension, heat treatment, hardness, NDT |
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Quality |
ISO9001, PPAP control production |
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Lead time |
3-20 days for sample, consult with customer for mass production |
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Delivery |
Express for sample, ocean shipping/air for mass production |
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Our Capabilities |
CNC machining, CNC center machining, drilling, milling, turning, grinding, tapping, boring |
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Engineering & manufacturing services |
R&D process, tooling and gauging design, product design Integrated CAD/CAM system, test and measuring |
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Others |
Recyclable, in line with environmental requirements and standards, and reliable |
C. Construction
Construction by machining
D Workmanship
The workmanship have good production practice. Care are taken to ensure High accuracy and smooth surfaces
E. Certificates
- Material certificates
- Measuring reports
- Inspection protocol
- NDT reports
- Fixed certificate: ISO9001 certificate
Photos of our machining
Our service
Besides above the general specifications, we can also supply some specific machining parts as clients' requirements.
Package and shipping
Export cartons, pallets, wooden cases, Polybags
container package and container transportation
CNC Other Parts,Cnc Machine Parts,Cnc Lathe Machine Components ,Cnc Precision Machine Parts
Shinvast Industry Ltd , http://www.shinvastindustry.com